Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep
SIGMA/F2883 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Mouse IgG FITC
MDL Number: MFCD00162642
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:128 |
| Application: | Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) |
| Biochem/physiol Actions: | Binds all mouse Igs. Useful when trying to avoid background staining due to the presence of Fc receptors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is specific for ) F(ab′)2 fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors. |
| Immunogen: | Mouse IgG |
| Immunogen: | Pepsin digest of antiserum |
| Other Notes: | Antibody adsorbed with human serum proteins. |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1, 2 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |