Fluorescein di(β-D-galactopyranoside)
SIGMA/F2756
Synonym: FDG
CAS Number: 17817-20-8
Empirical Formula (Hill Notation): C32H32O15
Molecular Weight: 656.59
MDL Number: MFCD00077344
Linear Formula: C32H32O15
Product Type: Chemical
| assay | ≥98% (HPLC) |
| form | powder |
| InChI | 1S/C32H32O15/c33-11-21-23 |
| InChI key | ZTOBILYWTYHOJB-WBCGDKOGSA |
| Quality Level | 100 ![]() |
| SMILES string | OC[C@H]1O[C@@H](Oc2ccc3c( |
| solubility | methanol: 20 mg/mL, clear, yellow |
| storage temp. | −20°C |
| Application: | Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting). |
| Application: | Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay. |
| Biochem/physiol Actions: | Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene. |
| General description: | Fluorogenic substrate for β-galactosidase |
| Packaging: | 1, 5, 25 mg in glass insert |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352201 |

