Ficoll® 400
SIGMA/F2637 - BioXtra, Molecular Biology, lyophilized powder
Synonym: Polysucrose 400; Ficoll 400
CAS Number: 26873-85-8
MDL Number: MFCD00081599
Product Type: Chemical
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| biological source | non-animal source |
| foreign activity | DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected |
| form | lyophilized powder |
| grade | Molecular Biology |
| product line | BioXtra |
| Quality Level | 200  |
| storage temp. | room temp |
| Application: | Suitable for • hybridization experiments to reduce non-specific binding of labeled probes to the membrane • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood) • producing density gradients to separate subcellular components • vitrification of cell and tissue samples. • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV). |
| Application: | Used for cell separation and organelle isolation. |
| Features and Benefits: | • free from DNases, RNases, phosphatases and proteases • preserves functional and morphological integrity of the separated cells |
| General description: | Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation. |
| Legal Information: | Ficoll is a registered trademark of Cytiva |
| Other Notes: | A nonionic synthetic polymer of sucrose. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H350 |
| Precautionary statements | P202 - P261 - P264 - P280 - P302 + P352 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |