Fibronectin human foreskin fibroblasts
SIGMA/F2518 - lyophilized powder, suitable for cell culture
Synonym: Fibronectin
EC Number: 289-149-2
MDL Number: MFCD00131062
Product Type: Chemical
Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound peptides of molecular weight 220 kDa.
SDS-PAGE Fibronectin Human foreskin fibroblasts (Cat. No. F2518) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Fibronectin Human foreskin fibroblasts that elicits a 50% attachment factor in cell based bioassay.
| binding specificity | Peptide Source: Collagen |
| biological source | human foreskin fibroblasts |
| form | lyophilized powder |
| impurities | Human Source, none detected (HbSAg) |
| Human Source, none detected (HCV) | |
| Human Source, none detected (HIV) | |
| Human Source, none detected (HTLV) | |
| Microbial Contamination, passes test | |
| mol wt | ~550 kDa |
| NCBI accession no. |   |
| packaging | pkg of 0.5 mg |
| product line | BioReagent |
| Quality Level | 200 |
| shipped in | ambient |
| solubility | H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.) |
| storage temp. | −20°C |
| surface coverage | 1‑5 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02751 |
| Analysis Note: | Protein homogeneity is evaluated by immunoelectrophoresis. |
| Application: | Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells |
| Application: | Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium. |
| Biochem/physiol Actions: | Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment. |
| Disclaimer: | Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended. |
| Features and Benefits: | • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium. • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts. • The starting material is tested and found negative for HIV and HBsAg. • The product is soluble in sterile water, yielding a clear, colorless solution. • It can be stored at -20°C for at least three years. |
| General description: | Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms. |
| Packaging: | 0.5 mg in serum bottle |
| Physical form: | Lyophilized in CAPS buffered saline |
| Preparation Note: | dialyzed against chloroform |
| Preparation Note: | Store reconstituted solution in working aliquots at -20°C or lower. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |