Anti-FKHRL1 (FOXO3a) antibody produced in rabbit
SIGMA/F2178 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AF6q21; Anti-FKHRL1; Anti-FKHRL1P2; Anti-FOXO2; Anti-FOXO3A
MDL Number: MFCD03791550
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of human breast carcinoma stained with 10 μg/mL Anti-FKHRL1 (FOXO3a) antibody produced in Rabbit (Cat. No. F2178) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:200 Anti-FKHRL1 (FOXO3a) antibody produced in Rabbit (Cat. No. F2178). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No.F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of 29T exp human FKHRL1 was separated on SDS-PAGE and probed with 1:2500 Anti-FKHRL1 (FOXO3a) antibody produced in Rabbit (Cat. No. F2178). The antibody was developed using Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. 1:2,500 antibody 2. 1:5,000 antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:200 using paraformaldyde-fixed FKHRL1 transfected 293T cells |
| microarray: suitable | |
| western blot: 1:2,500 using human FKHRL1 in transfected 293T cell extracts | |
| UniProt accession no. | O43524  |
| Application: | Anti-FKHRL1 (FOXO3a) antibody produced in rabbit has been used in: • immunohistochemistry • western blotting • immunofluorescence staining |
| Biochem/physiol Actions: | Forkhead transcription factor superfamily proteins act as the targets of the phosphoinositide-3-kinase |
| Biochem/physiol Actions: | Forkhead1 (FOXO-3A or FKHRL-1) functions to protect cells from oxidative stress . Dephosphorylation of FOXO3a stimulates the Fas ligand gene that activates apoptosis in cells . Anti-FKHRL1 is specific for human and mouse FKHRL1. FKHRL1 immunizing peptide specifically inhibits the staining of FKHRL1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Forkhead family transcription factor (FKHRL1), also known as FOX3a, is a member of the forkhead transcription factor superfamily. In FKHRL1 (FOX3a), there are three PKB phosphorylation consensus sites, Thr32, Ser253, and Ser315. Thr32 and Ser253 are phosphorylated by PKB after induction by survival factors such as IGF-1. |
| Immunogen: | thetic peptide corresponding to the N-terminus of human FKHRL1 (amino acids 653-668 with N-terminally added cystein) conjugated to maleimide-activated KLH. This sequence is conserved in human and mouse FKHRL1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |