Anti-α-Tubulin−FITC antibody, Mouse monoclonal
SIGMA/F2168 - clone DM1A, purified from hybridoma cell culture
Synonym: Monoclonal Anti-α-Tubulin
Product Type: Chemical
Proximity Ligation Assay JOURNAL CITATION: IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. By: Taulet, N., Vitre, B., et al. in Nat Commun, 2017. PubMed ID: 29203870 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-017-01479-3), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: NMNAT2-mediated NAD+ generation is essential for quality control of aged oocytes. By: Wu, X., Hu, F., et al. in Aging Cell, 2019. PubMed ID: 30909324 Image collected and cropped by CiteAb from the following publication, (https://onlinelibrary.wiley.com/doi/abs/10.1111/acel.12955), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: LIMK2 mediates resistance to chemotherapeutic drugs in neuroblastoma cells through regulation of drug-induced cell cycle arrest. By: Gamell, C., Schofield, A. V., et al. in PLoS One, 2013. PubMed ID: 23991158 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0072850), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Chicken fibroblast cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Anti-α-Tubulin-FITC antibody, Mouse monoclonal (Cat. No. F2168).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | DM1A, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | yeast, mouse, amphibian, human, rat, chicken, fungi, bovine |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:50 using cultured BHK cells |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-α-Tubulin antibody is suitable for immunostaining of: • respiratory epithelium tissue in a study to determine the tubulin expression in the mice cilia • breast cancer tissue sections to study the effect of LMO4 on the centrosome amplification and mitotic spindle abnormalities • spindle and chromosomes of oocytes Mouse monoclonal antibody has been used in the: • detection of tubulin by immunofluorescence and confocal microscopy in lung carcinoma cell. • immunofluorescent staining of microtubules in human embryos and mitotic spindles from spleen lymphoblast. |
| Biochem/physiol Actions: | The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. |
| Biochem/physiol Actions: | The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco. |
| Biochem/physiol Actions: | The N-terminal domain of α−tubulin binds GTP, which is non-exchangeable mechanism, whereas highly acidic C-terminal domain is capable to bind microtubule-associated proteins (MAPs). Both domains are associated with the microtubular self-assembly. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. Mutations in tubulin α-4A gene leads to a neurodegenerative disease called amyotrophic lateral sclerosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α/β-Tubulin, the major building block of microtubules, belongs to the tubulin superfamily of proteins. They are ubiquitously found in all eukaryotic cells. It exists in multiple isotype in many organisms.† It is a heterodimer with intracellular, hollow, cylindrical, filamentous structures. It is located on human chromosome 2q35. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β--Tubulin is a heterodimer, which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. Tubulin is a highly conserved protein with two terminal domains: large N and C terminal. Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. |
| Immunogen: | microtubules from chicken embryo brain. |
| Immunogen: | Microtubules from chicken embryo brain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide |
| Preparation Note: | For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing , or storage in "frost-free" freezers. is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |