Monoclonal ANTI-FLAG® M2
SIGMA/F1804 - 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Synonym: flag antibody; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
Immunofluorescence
MDCK canine kidney epithelial cells, transfected with FLAG tagged myr-PKCz are labeled with Monoclonal ANTI-FLAG M2® (Cat. No. F1804) (red). Actin is stained using phalloidin (blue) and endogenous RhoGDI is labeled green.
From Federica Chianale, Clinical and Experimental Medicine, University of Piemonte Orientale, Novara, Italy.
MDCK canine kidney epithelial cells, transfected with FLAG tagged myr-PKCz are labeled with Monoclonal ANTI-FLAG M2® (Cat. No. F1804) (red). Actin is stained using phalloidin (blue) and endogenous RhoGDI is labeled green.
From Federica Chianale, Clinical and Experimental Medicine, University of Piemonte Orientale, Novara, Italy.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| purified by | affinity chromatography (affinity tag purification) |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| Application: | For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems. Western Blotting and EIA Learn more product details in our FLAG® application portal. |
| Biochem/physiol Actions: | Binding site:N-Asp-Tyr-Lys-Asp-As |
| General description: | The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus Method of purification - Affinity tag purification |
| Immunogen: | FLAG; peptide sequence DYKDDDDK |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Contains 50% glycerol in phosphate buffered saline. |
| Preparation Note: | Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |