Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit
SIGMA/F1763 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Rat IgG (whole molecule)
MDL Number: MFCD00162811
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human tonsil tissue sections were stained with 1:100 Anti-Human IgG antibody produced in Rat, followed by 1:320 Anti-Rat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F1763).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:25 Anti-Human IgG antibody produced in Rat, followed by 1:320 Anti-Rat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F1763).
Flow Cytometry 1×106 HUVEC cells were labeled with 1 μg/test Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal, Clone: RCR-252 (Cat. No. E6280) followed by Anti-Rat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F1763). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 |
| indirect immunofluorescence: 1:320 |
| Application: | Anti-Rat IgG (whole molecule)-FITC antibody has been used: • in immunolabelling for confocal laser scanning microscopy (CLSM) • in immunostaining |
| Application: | Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Flow cytometry/Cell sorting (1 paper) Immunofluorescence (1 paper) |
| Application: | Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody. |
| Biochem/physiol Actions: | IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen. |
| General description: | Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm. |
| General description: | Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins. |
| Immunogen: | Purified rat IgG |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |