Fumarase from porcine heart
SIGMA/F1757 - ammonium sulfate suspension, ≥300 units/mg protein (biuret)
Synonym: L-Malate hydrolyase; Fumarate hydratase
CAS Number: 9032-88-6
EC Number: 232-887-7
MDL Number: MFCD00131111
Product Type: Chemical
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| foreign activity | malic dehydrogenase ≤0.1% |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥300 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Fumarase from porcine heart has been used: • as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay • for immobilized enzyme based stability assay • in fumarate hydratase assays in human lung carcinoma cells (A549) |
| Application: | Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells. |
| Biochem/physiol Actions: | Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism. |
| Biochem/physiol Actions: | Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome. |
| General description: | Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes. |
| General description: | Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family. |
| Other Notes: | One unit will convert 1.0 μmole of |
| Physical form: | Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol |
| Preparation Note: | Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥300 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.2.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |