Anti-Fibronectin antibody, Mouse monoclonal
SIGMA/F0916 - clone IST-4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Fibronectin antibody produced in mouse; Anti-CIG; Anti-ED-B; Anti-FINC; Anti-FN; Anti-FNZ; Anti-GFND; Anti-GFND2; Anti-LETS; Anti-MSF; Anti-SMDCF
MDL Number: MFCD00162291
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells. By: Hunter, M. C., O′Hagan, K. L., et al. in PLoS One, 2014. PubMed ID: 24466266 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0086842), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells. By: Hunter, M. C., O′Hagan, K. L., et al. in PLoS One, 2014. PubMed ID: 24466266 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0086842), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells. By: Hunter, M. C., O′Hagan, K. L., et al. in PLoS One, 2014. PubMed ID: 24466266 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0086842), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting Whole extract of Human forskin fibroblasts HS-68 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Fibronectin antibody produced in Mouse, Clone: IST-4 (Cat. No. F0916). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:250 antibody 2. 1:500 antibody 3. Negative Control
Immunofluorescence HS-68 human foreskin fibroblast cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:100 Monoclonal Anti-Fibronectin antibody produced in mouse clone: IST-4 (Cat. No. F0916). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat (Cat. No. F5262).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | IST-4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 240 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rabbit, mouse, goat, bovine, canine, chicken |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:100 using cultured human fibroblasts |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| radioimmunoassay: suitable | |
| western blot: 1:500-1000 using human fibroblasts cell extract | |
| UniProt accession no. | P02751  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Fibronectin antibody has been used : • in immunohistochemistry • in confocal microscopy • in immunocytochemistry • western blotting analysis |
| Application: | Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by pepsin but not by trypsin). |
| Biochem/physiol Actions: | Fibronectin participates in growth, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in the formation of extracellular matrix. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN’s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells. |
| General description: | Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. |
| General description: | Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody recognizes an epitope located within the 5th type III repeat of human plasma fibronectin. This epitope is common to all fibronectin forms. The antibody localizes plasma and cellular fibronectin both in its native and denatured-reduced forms (240 kDa) applying immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. The product reacts with human fibronectin. No reactivity is observed with other species including goat, bovine, dog, rabbit, mouse and chicken. |
| Immunogen: | human plasma fibronectin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |