Anti-Human IgG1−FITC antibody, Mouse monoclonal
SIGMA/F0767 - clone 8c/6-39, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG1 (Fc specific)
Product Type: Chemical
Particle Immunofluorescence Agarose beads coated with Human IgG probed and developed using 1:32 Monoclonal Anti-Human IgG1-FITC antibody produced in mouse, Clone: clone 8c/6-39 (Cat. No. F0767).
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | 8c/6-39, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot immunobinding: 1:64 |
| particle immunofluorescence: 1:32 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Flow cytometry/Cell sorting (1 paper) |
| Application: | Monoclonal Anti-Human IgG1−FITC antibody produced in mouse has been used in flow cytometry. It has also been used in direct hemagglutination (HA) and hemagglutination inhibition |
| Biochem/physiol Actions: | IgG1 and IgG3 have an ability to adhere to the mononuclear phagocytes. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. |
| General description: | Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study. |
| Physical form: | Solution in 0.01 M sodium phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |