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Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)

SIGMA/F0628 - lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

Synonym: Ferridoxin-NADP+ oxidoreductase

CAS Number: 9029-33-8
MDL Number: MFCD00131050
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-F0628-1UN 1 unit
$150.00
1/EA
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45-F0628-5UN 5 units
$519.00
1/EA
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45-F0628-10UN 10 units
$942.00
1/EA
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foreign activity NADH diaphorase activity ≤10 units/mg solid
form lyophilized powder
Quality Level 200 
specific activity ≥15 units/mg solid
storage temp. −20°C
Application: Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Application: Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.
Biochem/physiol Actions: Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Biochem/physiol Actions: Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
General description: Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.
Other Notes: One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥15 units/mg solid
Storage Temp. −20°C
Enzyme Commission (EC) Number 1.18.1.2   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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