Monoclonal Anti-CD3−FITC antibody produced in mouse
SIGMA/F0522 - clone UCHT-1, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-CD3
Product Type: Chemical
Flow Cytometry 1×106 Human lymphocytes were labeled with 10 μg/test Anti-CD3-FITC antibody, Mouse monoclonal, Clone: UCHT-1 (Cat. No. F0522). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
Flow Cytometry 1×106 Human monocytes were labeled with 10 μg/test Anti-CD3-FITC antibody, Mouse monoclonal, Clone: UCHT-1 (Cat. No. F0522). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
Flow Cytometry 1×106 Human granulocytes were labeled with 10 μg/test Anti-CD3-FITC antibody, Mouse monoclonal, Clone: UCHT-1 (Cat. No. F0522). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | UCHT-1, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 10 μL using 1 × 106 cells |
| UniProt accession no. | P07766  |
| Application: | Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay. |
| Biochem/physiol Actions: | Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471 4th Workshop: paper no. T3.2 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation. |
| General description: | The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor. |
| Immunogen: | human thymocytes followed by Sezary T cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |