Fast Blue RR Salt
SIGMA/F0500 - crystalline
Synonym: 4-
CAS Number: 14726-29-5
Empirical Formula (Hill Notation): C15H14ClN3O3 · 0.5ZnCl2
Molecular Weight: 387.89
EC Number: 238-777-5
MDL Number: MFCD00074762
Linear Formula: C15H14ClN3O3 ·
Product Type: Chemical
ATR-IR Spectra for 4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F0500-25G
| ε (extinction coefficient) | ≥15000 at 326-332 nm |
| ≥7000 at 390-396 nm | |
| application(s) | diagnostic assay manufacturing hematology histology |
| form | crystalline |
| InChI | 1S/2C15H13N3O3.4ClH.Zn/c2 |
| InChI key | LLARVKAOQLUCQI-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Cl-].[Cl-].Cl[Zn]Cl.COc1 |
| solubility | water: 1 mg/mL, clear to hazy, yellow-green |
| storage temp. | −20°C |
| technique(s) | microbe id | staining: suitable |
| Application: | Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red. |
| Biochem/physiol Actions: | Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue. |
| Packaging: | 25 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Colour Index Number | 37155 |
| UNSPSC | 12171500 |