Fas Ligand human
SIGMA/F0427 - >95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder
Synonym: FasL
MDL Number: MFCD01092558
Product Type: Chemical
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| assay | >95% (SDS-PAGE) |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 26-28 kDa by SDS-PAGE |
| monomer calculated mol wt ~18 kDa | |
| Quality Level | 100  |
| recombinant | expressed in CHO cells |
| storage temp. | −20°C |
| UniProt accession no. | P48023  |
| Analysis Note: | Measured by its ability to induce apoptosis in Jurkat cells. |
| Application: | Fas Ligand (FASLG) human has been used for- • the study of FASLG stimulation in luteal cells obtained from mid-corpus luteum (CL) and • the study of apoptosis induction and intracellular caspase-3 assay in human cells. |
| Biochem/physiol Actions: | Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1). |
| Biochem/physiol Actions: | FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichlo |
| Biochem/physiol Actions: | Protein belonging to the tumor necrosis factor (TNF) family of cytokines; induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1). |
| General description: | FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis. |
| Other Notes: | Human Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acids 134-281. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |