Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal
SIGMA/F0397 - clone PCK-26, purified from hybridoma cell culture
Synonym: Anti-
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 1:50 Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in Mouse, Clone: PCK-26 (Cat. No. F0397).
Immunohistochemistry Formalin fixed, paraffin-embedded Human skin tissue sections were stained with 1:50 Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in Mouse, Clone: PCK-26 (Cat. No. F0397).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PCK-26, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues |
| immunohistochemistry (frozen sections): suitable |
| Application: | Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse has been used in: • immunohistochemistry • staining chicken intestinal cells • immunofluorescence staining |
| Application: | Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse is suitable for direct immunofluorescence at a dilution of 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues and immunohistochemistry (frozen sections). |
| Biochem/physiol Actions: | The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type. |
| General description: | FITC Monoclonal Anti-Pan Cytokeratin is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal anti-pan cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II, neutral-to-basic subfamily. Cytokeratin peptide 5 is the primary type II keratin in stratified epithelia while cytokeratin type 8 is a major type II keratin in simple epithelia. Cytokeratin 6 is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. |
| Immunogen: | cytokeratin from human epidermis. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |