Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit
SIGMA/F0300 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FGFR-2
MDL Number: MFCD02096344
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit (Cat. No. F0300) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Fibroblast Growth Factor Receptor-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with 1:10,000 Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.5 μg/mL.
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 2 μg/mL Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit (Cat. No. F0300) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of HEK-293T cells expressing FGFR-2 were separated on SDS-PAGE and probed with Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit (Cat. No. F0300). The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody 3. 0.5 μg/mL antibody untransfected 293T cells 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 115-125 kDa (doublet) |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections |
| western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells | |
| UniProt accession no. | P21802  |
| Application: | Anti-Fibroblast Growth Factor Receptor-2 has been used in immunoblotting, immunoprecipitation, immunohistochemistry,west |
| Application: | Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit was used for immunohistochemistry of free-floating rat brain tissue sections. |
| Biochem/physiol Actions: | Fibroblast growth factors (FGFs) and their receptors, FGFRs, are important regulators of cell proliferation, migration, tissue repair and differentiation during vertebrate development. FGFR-2 isoforms are expressed exclusively on cells of epithelial origin and are involved in tumor progression in thyroid cancers. Abnormalities in FGFR-2 expressions have been observed in skin, bone and cancer pathologies. |
| Biochem/physiol Actions: | Fibroblast growth factors receptors (FGFRs) mediated signal transduction requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Mutations in the extracellular domain of FGFR-2 causes Crouzon, as well as other craniosynostosis syndromes (e.g. Pfeiffer′s, Apert′s, Jackson-Weiss′), disorders of human skeletal development. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibroblast growth factors receptors (FGFRs) are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 k Da proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid ′acidic box′, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. At the mRNA level, FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells. |
| Immunogen: | synthetic peptide corresponding to amino acids 809-821 of the cytoplasmic region of human FGFR-2 (bek). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |