hBFGF
SIGMA/F0291 - FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
Synonym: Fibroblast Growth Factor-Basic human; FGF2; Fibroblast Growth Factor-Basic human; bFGF; hBFGF; FGF2; bFGF
CAS Number: 106096-93-9
MDL Number: MFCD00081382
Product Type: Chemical
SDS-PAGE Fibronectin Proteolytic Fragment from human plasma (Cat. No. F0291) was assessed on SDS-PAGE.
Bioassay Basic growth factor is a protein that is mitogenic for a variety of cell types. hBFGF growth factor potency is quantified by its ability to stimulate a growth of Balb/3T3 cells in serum free conditions. The relative cell growth is detected using a fluorimetric assay based on the measurement of the conversion of Resazurin to Resorufin by metabolically active cells. The amount of hBFGF that will give 50% of the maximal proliferation (EC50) is calculated from the different amounts assayed.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| color | white to faint yellow cast |
| form | lyophilized powder |
| impurities | ≤1.00 EU/μg |
| mol wt | 16.0 kDa |
| packaging | pkg of 25 μg |
| pkg of 4X25 μg | |
| potency | ≤-0.6 ng/mL |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.025 mg, clear, colorless to faintly yellow |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P09038  |
| Analysis Note: | The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin. |
| Application: | Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC). |
| General description: | Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells. |
| Physical form: | Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |