Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat
SIGMA/F0257 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Mouse Fitc; Anti-Mouse Fitc - Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat
MDL Number: MFCD00162642
Product Type: Chemical
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Fluorescent micrographs of mitotic newt lung cells. Microtubules are stained with Monoclonal Anti-β-Tubulin and Goat Anti-Mouse IgG FITC conjugate (Cat. No. F0257). Chromosomes are counterstained with Hoechst 33342. The astral microtubules are green, the spindle is yellow and the chromosomes are blue.
From C. Rieder, Wadsworth Center, Division of Molecular Medicine, Lab of Cell Regulation, Albany, NY.
Immunofluorescence Cytomegalovirus (CMV) infected fibroblast cells labeled with Monoclonal Anti-CMV followed by Goat Anti-Mouse IgG-FITC conjugate (Cat. No. F0257). From F. Brady, Med Immune, Inc., Gaithersburg, MD.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:32 |
| Application: | Anti-Mouse IgG (whole molecule)-FITC antibody has been used in: • immunohistochemistry (1:200) (1:500) • immunofluorescence (1:100)(1:500)(1:200) • immunocytochemistry(1:1 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to foetus through the placenta that is vital for immune defense of the neonate against infections. Mouse IgGs has four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. |
| Immunogen: | Purified mouse IgG |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1, 2 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |