Fructose-6-phosphate Kinase from Bacillus stearothermophilus
SIGMA/F0137 - Type VII, lyophilized powder, ≥50 units/mg protein
Synonym: 6-
CAS Number: 9001-80-3
EC Number: 232-633-5
MDL Number: MFCD00131107
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 34 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| type | Type VII |
| Application: | Fructose-6-phosphate kinase from Bacillus stearothermophilus has been used: • in steady state analysis of phosphofructokinase activity in the presence of ATP deuterated at the C8 position(C8-D ATP) • for standard curve generation for quantifying muscle phosphofructokinase (PFK) activity |
| Application: | Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle. |
| Application: | Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction). |
| Biochem/physiol Actions: | Fructose-1,6-bisphosphata |
| Biochem/physiol Actions: | Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs). |
| General description: | Fructose-6-phosphate kinase from Bacillus stearothermophilus, a 34 kDa enzyme, belongs to phosphofructokinase (PFK) - like superfamily. The glutamate 161 and arginine 162 residues at the active site are crucial for substrate binding. |
| General description: | Research Area: Cell Signaling Bacillus stearothermophilus phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface. |
| Other Notes: | One unit will convert 1.0 μmole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 °C. |
| Packaging: | 100, 250 units in glass bottle |
| Physical form: | Lyophilized powder containing buffer salt (e.g. phosphate buffer, or Tris buffer with NaCl) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |