eSpCas9 Protein
SIGMA/ESPCAS9PRO - from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS
Synonym: Enhanced specificity Cas9; eCas9; eSpCas9; eSpCas9 1.1; eSpyCas9
Product Type: Chemical
Ribonucleoprotein (RNP) complex consisting of enhanced specificity SpCas9 and gRNA. For use with either two-part (crRNA:tracrRNA) or one-part (sgRNA) SygRNA™ synthetic gRNA.
| application(s) | CRISPR |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| packaging | pkg of 1 kit (3 components) |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| storage temp. | −20°C |
| Application: | Functional Genomics/Target Validation/Genome Editing |
| Biochem/physiol Actions: | Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency. |
| Features and Benefits: | • Enhanced specificity compared to wild type Cas9 • Highly active • Ready-to-inject/transfe |
| General description: | Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection. |
| Legal Information: | CRISPR Use License Agreement |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Each kit consists of: • one vial of eSpCas9 recombinant protein • one vial containing 1 mL of 1× dilution buffer • one vial containing 1 mL of nuclease-free water with glycerol |
| Other Notes: | Use our CRISPR Selection Tool to order gRNA Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRpr |
| Packaging: | pkg of 50 μg pkg of 250 μg |
| Preparation Note: | Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |
| Components | Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli; Dilution buffer for Cas9 proteins; Reconstitution solution for Cas9 proteins |