eSpCas9-Blasticidin Lenti Plasmid
SIGMA/ESPCAS9BST
Product Type: Chemical
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| concentration | 20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA) |
| packaging | vial of 50 μL |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| selection | ampicillin |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Functional Genomics/Target Validation • Creation of gene knockouts in multiple cell lines • Complete knockout of genes not amenable to RNAi • Manufacture of eSpCas9 expressing Lentiviral Particles |
| Biochem/physiol Actions: | CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency. |
| Features and Benefits: | • Enhanced specificity compared to wild type Cas9 • Highly Active • Ready to use purified plasmid DNA |
| General description: | This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of eSpCas9 and a blasticidin resistance cassette linked by a 2A peptide (EF1a-eSpCas9-2A-Blastici To order gRNA in any format click here |
| Legal Information: | CRISPR Use License Agreement Lentiviral, WPRE and Evrogen Fluorophore Licenses |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |