Ethylenediaminetetraacetic acid disodium salt dihydrate

SIGMA/E5134 - suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

Synonym: Sequestrene Na₂; Disodium ethylenediaminetetraacetate dihydrate; EDTA disodium salt; EDTA-Na₂; Edathamil; Edetate disodium salt dihydrate; Sequestrene Na₂

CAS Number: 6381-92-6
Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
Molecular Weight: 372.24
EC Number: 205-358-3
Linear Formula: C₁₀H₁₄N₂Na₂O₈ · 2H₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E5134-50G	50 g	$48.20 1/EA	Add To Favorites
45-E5134-100G	100 g	$73.30 1/EA	Add To Favorites
45-E5134-250G	250 g	$145.00 1/EA	Add To Favorites
45-E5134-500G	500 g	$202.00 1/EA	Add To Favorites
45-E5134-1KG	1 kg	$270.00 1/EA	Add To Favorites
45-E5134-5KG	5 kg	$1070.00 1/EA	Add To Favorites

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This image depicts SKU: E5134-100G

Properties
Descriptions
Safety Info.
Basic Data

assay	99.0-101.0% (titration)
cation traces	Fe: ≤0.005%
	Pb: ≤0.002%
foreign activity	DNase, RNase, NICKase and protease, none detected
form	powder
grade	Molecular Biology
impurities	≤0.005% Insolubles
	≤0.1% Nitrilotriacetic acid (NTA)
InChI	1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;21H2/q;2+1;;/p-2
InChI key	OVBJJZOQPCKUOR-UHFFFAOYSA-L
mp	248 °C (dec.) (lit.)
Quality Level	300
reaction suitability	reagent type: chelator
SMILES string	O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
solubility	3 M NaOH: 1.6 g/10 mL
storage temp.	room temp
suitability	suitable for electrophoresis
	suitable for molecular biology
technique(s)	electrophoresis: suitable

Application:	Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Application:	EDTA disodium salt has been used in research:• as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more • an anticoagulant for whole blood collection • a stabilizer solution for freezing cell culture samples • in combination with PBS and trypsin, to trypsinize cells • for phytoextraction of heavy metals in polluted soils
Features and Benefits:	• Molecular Biology grade, suitable for many molecular biology applications • Free of endonucleases DNase, RNase, Nickase, and Protease
General description:	EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	5 kg in poly drum
Packaging:	50, 100, 250, 500 g in poly bottle

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H332 - H373 - H412
Precautionary statements	P260 - P271 - P273 - P304 + P340 + P312 - P314 - P501
Hazard Codes	Xn
Risk Statements	20-48/20
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	99.0-101.0% (titration)
mp	248 °C (dec.) (lit.)
Storage Temp.	room temp
UNSPSC	12352107

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