Earle’s Balanced Salts
SIGMA/E3024 - With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym: EBSS; EBSS
MDL Number: MFCD00217343
Product Type: Chemical
| components | phenol red: no NaHCO3: 2.2 g/L glucose: 1.0 g/L (Dextro) |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment. |
| General description: | Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6). |
| Other Notes: | Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352207 |