ExtrAvidin®−Peroxidase
SIGMA/E2886 - buffered aqueous solution
Synonym: ExtrAvidin HRP
MDL Number: MFCD00163600
Product Type: Chemical
ELISA assay utilizing a biotinylated antibody and ExtrAvidin®−Peroxidase detection.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) antibody produced in mouse followed by Biotin (Cat. No. B7151) and 1:100 of ExtrAvidin®-Peroxidase (Cat. No. C2886).
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | direct ELISA: 1:2,000 |
| dot blot: 1:4,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 |
| Application: | ExtrAvidin®-Peroxidase is suitable for the following applications: • Dot blot at a minimum dilution of 1:4,000 • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100) • Direct ELISA at a minimum dilution of 1:2,000 It has also been used for: • Immunoprecipitation and Immunobloting • Ra2I solid-phase binding assays • Southwestern blotting • Western blotting • Immunocytochemistry |
| Biochem/physiol Actions: | Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques. |
| Biochem/physiol Actions: | The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Affinity purified protein |
| Preparation Note: | Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |