ExtrAvidin®−FITC
SIGMA/E2761 - buffered aqueous solution
MDL Number: MFCD00163600
Product Type: Chemical
Laser scanning confocal image of the distribution of cytoskeletal actin in the cortical region of the oocyte in the developing follicle of giant silkmoth (Hyalophora carcopia). Stained with Monoclonal Anti-Actin, (clone no. KJ43A, PrCat. No. A1804) followed by Goat Anti-Mouse IgG-Biotin conjugate (Cat. No. B7264) and ExtrAvidin-FITC (Cat. No. E2761). From I. Sauman, Wesleyan Univ., Middletown, CT.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by Biotin (Cat. No. B0529) and by 20 MUg/ml ExtrAvidin®-FITC (Cat. No. E2761).
Immunofluorescence Mouse splenocytes cells were stained with Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B8520). The conjugate was developed using 1:200 ExtrAvidin®-FITC (Cat. No. E2761).
| conjugate | FITC conjugate |
| extent of labeling | 3-6 mol fluorochrome per mol protein |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues |
| indirect immunofluorescence: 1:200 |
| Application: | ExtrAvidin®-FITC is suitable for the following applications: • immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues. • Immunofluorescence† • competitive binding assay |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide |
| Preparation Note: | Affinity purified protein |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |