Endothelial cell growth supplement from bovine neural tissue
SIGMA/E2759 - ECGS, suitable for cell culture
Synonym: ECGS; Endothelial Mitogen
Product Type: Chemical
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| biological source | bovine neuroblastoma |
| color | tan to brown |
| form | powder |
| impurities | ≤20 EU/vial (LAL test) |
| packaging | pkg of 15 and 5X15 mg |
| potency | 2-200 μg/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| solubility | water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl) |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay. |
| Application: | Endothelial cell growth supplement from bovine neural tissue has been used as a supplement in Dulbecco′s modified Eagle′s medium supplement and F-12K media supplement for human umbilical vein endothelial cells culture. |
| Biochem/physiol Actions: | Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC). It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs). It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation. An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. |
| General description: | Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF). |
| Preparation Note: | ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |