ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
SIGMA/E1270 - liquid, BioReagent, suitable for cell culture
Synonym: Basement membrane extract (BME); EHS matrix
MDL Number: MFCD00283131
Product Type: Chemical
Neurite outgrowth of PC12 rat neuronal cells after 2-day growth on ECM Gel Matrix.
| concentration | 8-12 mg/mL |
| form | liquid |
| product line | BioReagent |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02468  |
| P02469 |
|
| P10493 |
|
| P19137 |
|
| P43407 |
|
| Q61001 |
|
| Q61092 |
|
| Q61789 |
| Application: | ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells. |
| Biochem/physiol Actions: | The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel. Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV. |
| Disclaimer: | For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours. |
| Other Notes: | ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization. Product contains 50 mg/L Gentamicin Sulfate. |
| Preparation Note: | This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |