DL-Dithiothreitol

SIGMA/D9779 - Molecular Biology, ≥98% (HPLC), ≥99% (titration)

Synonym: (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol; threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
Molecular Weight: 154.25
EC Number: 222-468-7
MDL Number: MFCD00004877
Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-D9779-250MG	250 mg	$31.10 1/EA	Add To Favorites
45-D9779-1G	1 g	$76.80 1/EA	Add To Favorites
45-D9779-5G	5 g	$227.00 1/EA	Add To Favorites
45-D9779-10G	10 g	$408.00 1/EA	Add To Favorites
45-D9779-25G	25 g	$835.00 1/EA	Add To Favorites
45-D9779-50G	50 g	$1330.00 1/EA	Add To Favorites

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This image depicts SKU: D9779-50G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
	≥99% (titration)
cation traces	heavy metals (as Pb): ≤5 ppm
foreign activity	DNase, RNase, protease, none detected
form	powder
grade	Molecular Biology
InChI	1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key	VHJLVAABSRFDPM-QWWZWVQMSA-N
mp	41-44 °C (lit.)
Quality Level	200
reaction suitability	reagent type: reductant
SMILES string	O[C@H](CS)[C@H](O)CS
solubility	H₂O: 50 mg/mL
storage temp.	2-8°C
suitability	suitable for molecular biology

Application:	An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Application:	DL-Dithiothreitol is used in protein samples to reduce the disulfide bonds before loading the sample onto the gel for Western Blot or SDS-PAGE It has been used in the procedure of in situ hybridization of mRNA. It has been used:• as a component for protein extraction in western blot analysis • to prepare sample lysis buffer for quantitative mass spectroscopy • as a kinase buffer component for enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions:	Dithiothreitol (DTT) is frequently utilized in biochemical preparations of thiol proteins and chemical peptide synthesis. DTT is also used in protein chemistry research on the folding of proteins and enzyme activity.
Features and Benefits:	• Suitable for molecular biology • RNase, DNase, Exonuclease, and Protease-free • High purity (HPLC ≥98%) • No heavy metal ≤5ppm
General description:	Dithiothreitol (DTT) is an excellent reagent for maintaining SH groups in a reduced state in proteins. It initiates the thiol-disulfide exchange reaction to completely reduce the intra-or inter-molecular disulfide bonds in biomolecules. DTT specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel and sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Hence, it is highly effective in use with buffers that quantitatively reduce disulfides. DTT is less pungent and less toxic in nature than 2-mercaptoethanol. It is also 7x more concentrated than beta-mercaptoethanol.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1, 5, 10, 25, 50 g in poly bottle
Packaging:	250 mg in poly bottle

Symbol	GHS05,GHS07
Signal word	Danger
Hazard statements	H302 - H315 - H318
Precautionary statements	P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Hazard Codes	Xn
Risk Statements	22-36/37/38
Safety Statements	26-36
RIDADR	UN 3335
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC); ≥99% (titration)
mp	41-44 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12161504

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