DAPI
SIGMA/D9542 - for nucleic acid staining
Synonym: 4′,6-Diamidino-2-phenylindole dihydrochloride; 2-
CAS Number: 28718-90-3
Empirical Formula (Hill Notation): C16H15N5 · 2HCl
Molecular Weight: 350.25
EC Number: 249-186-7
MDL Number: MFCD00012681
Linear Formula: C16H15N5 · 2HCl
Product Type: Chemical
Immunofluorescence A. HeLa cells nuclei stained with 1 μg/mL 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Cat. No. D9542) (Blue) 100× magnification. B. Heat-shock treated NIH-3T3 cells stained for cytoplasmic stress like granules with 2 μg/mL Rabbit Anti-FMR1 (C-ter) (Cat. No. F4055) followed by Goat Anti-Rabbit, Cy3 conjugate (Red) and nuclei counterstained with 2 μg/mL DAPI (Cat. No. D9542) (blue) 40× magnification.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D9542-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D9542-1MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D9542-50MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D9542-5MG
| ε (extinction coefficient) | 30 at 263 nm in H2O at 1 mM |
| assay | ≥98% (HPLC) |
| fluorescence | λex 340 nm; λem 488 nm (nur DAPI) |
| λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C16H15N5.2ClH/c17-15(1 |
| InChI key | FPNZBYLXNYPRLR-UHFFFAOYSA |
| Quality Level | 300  |
| SMILES string | Cl.Cl.NC(=N)c1ccc(cc1)-c2 |
| solubility | H2O: 20 mg/mL |
| PBS: insoluble | |
| storage temp. | 2-8°C |
| suitability | suitable for fluorescence |
| technique(s) | transfection: suitable |
| Application: | DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. |
| Application: | DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. |
| Application: | Suitable for • DNA staining in agarose gels • analysis of changes in DNA during apoptosis • detection of mycoplasma • photofootprinting of DNA • immunofluorescent staining of cells DAPI has been used:- • in rapid monitoring of microbial contamination • in chromosomal banding technique • in detection of apoptotic cells • in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA • to stain mature pollen grains(0.5 mg/ml) |
| Biochem/physiol Actions: | Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. |
| Disclaimer: | Protect from light. |
| General description: | DAPI (4′,6-Diamidino-2-phenyli |
| General description: | heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 1, 5, 10, 50 mg in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H335 |
| Precautionary statements | P261 - P264 - P271 - P272 - P280 - P302 + P352 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |