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Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

SIGMA/D8900 - With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym: DMEM Hams F12; DME/F-12, 1:1 mixture

MDL Number: MFCD00217417
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-D8900-10X1L 1 L
$126.00
10/EA
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45-D8900-10L 10 L
$118.00
1/EA
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45-D8900-50L 50 L
$451.00
1/EA
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components sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
form powder
Quality Level 500 
shipped in ambient
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

• as a component of the culture medium for endometrial explants in vitro culture
• to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
• to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note: Formulated to contain 15.6 grams of powder per liter of medium.
Preparation Note: Supplement with 1.2 g/L sodium bicarbonate.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Storage Temp. 2-8°C
UNSPSC 12352207

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