Dimethyl sulfoxide
SIGMA/D8418 - Molecular Biology
Synonym: Methyl Sulfoxide; Methylsulfinylmethane; Molecular Biology Grade DMSO; DMSO
CAS Number: 67-68-5
Empirical Formula (Hill Notation): C2H6OS
Molecular Weight: 78.13
EC Number: 200-664-3
MDL Number: MFCD00002089
Linear Formula: (CH3)2SO
Product Type: Chemical
Representative image of product packaging.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8418-100ML.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8418-1L.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8418-500ML.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8418-50ML
| absorption | passes test |
| assay | ≥99.9% |
| autoignition temp. | 573 °F |
| bp | 189 °C (lit.) |
| color | colorless |
| density | 1.10 g/mL (lit.) |
| expl. lim. | 42 %, 63 °F |
| foreign activity | DNase and RNase, none detected |
| form | liquid |
| grade | Molecular Biology |
| impurities | ≤0.001 meq/g Titratable acid |
| ≤0.1% water (Karl Fischer) | |
| InChI | 1S/C2H6OS/c1-4(2)3/h1-2H3 |
| InChI key | IAZDPXIOMUYVGZ-UHFFFAOYSA |
| mp | 16-19 °C (lit.) |
| product line | BioReagent |
| Quality Level | 200  |
| refractive index | n |
| shelf life | Recommended retest period - 2 years |
| SMILES string | CS(C)=O |
| solubility | H2O: miscible (completely) |
| storage temp. | room temp |
| suitability | suitable for molecular biology |
| technique(s) | DNA sequencing: suitable |
| PCR: suitable | |
| transfection: suitable | |
| vapor density | 2.7 (vs air) |
| vapor pressure | 0.42 mmHg ( 20 °C) |
| Application: | DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in • polymerase chain reaction (PCR) • amplification of cDNA libraries • DNA sequencing • column-loading buffers for poly(A)+ RNA selection • buffers for the transformation of competent E. coli • transfection protocols. Dimethyl sulfoxide has been used- • as an oligonucleotide solvent in array spotting[1] • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR) • for cell lysis during MTT (3-(4,5-dimethylthiazol-2 • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetri • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent. To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended. |
| Disclaimer: | Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. |
| Features and Benefits: | This product is free from DNases, RNases, phosphatases and proteases. |
| General description: | Features and Benefits: • Suitable for Molecular Applications • Nuclease-Free DMSO • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free • Molecular Biology Grade DMSO Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals. This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 1 L in glass bottle |
| Packaging: | 50, 100, 250, 500 mL in glass bottle |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Purity | ≥99.9% |
| bp | 189 °C (lit.) |
| mp | 16-19 °C (lit.) |
| Density | 1.10 g/mL (lit.) |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 12191502 |