5,5′-Dithiobis(2-nitrobenzoic acid)

SIGMA/D8130 - ≥98%, BioReagent, suitable for determination of sulfhydryl groups

Synonym: 3-Carboxy-4-nitrophenyl disulfide; 6,6′-Dinitro-3,3′-dithiodibenzoic acid; Bis(3-carboxy-4-nitrophenyl) disulfide; DTNB; Ellman’s Reagent

CAS Number: 69-78-3
Empirical Formula (Hill Notation): C₁₄H₈N₂O₈S₂
Molecular Weight: 396.35
EC Number: 200-714-4
MDL Number: MFCD00007140
Linear Formula: [-SC₆H₃(NO₂)CO₂H]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-D8130-500MG	500 mg	$31.50 1/EA	Add To Favorites
45-D8130-1G	1 g	$56.60 1/EA	Add To Favorites
45-D8130-5G	5 g	$139.00 1/EA	Add To Favorites
45-D8130-10G	10 g	$257.00 1/EA	Add To Favorites
45-D8130-25G	25 g	$516.00 1/EA	Add To Favorites
45-D8130-100G	100 g	$1990.00 1/EA	Add To Favorites

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This image depicts SKU: D8130-25G

12 Principles of Green Chemistry: Principle 2—Atom Economy. This product was designed to maximize the incorporation of all raw materials used in the manufacturing process.

12 Principles of Green Chemistry: Principle 7—A raw material or feedstock should be renewable rather than depleting whenever technically and economically practicable.

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98%
form	powder
InChI	1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI key	KIUMMUBSPKGMOY-UHFFFAOYSA-N
mp	240-245 °C (dec.) (lit.)
product line	BioReagent
Quality Level	200
SMILES string	OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
suitability	suitable for determination of sulfhydryl groups

Application:	Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.
General description:	5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.
Packaging:	1, 5, 10, 25, 100 g in poly bottle
Packaging:	500 mg in glass insert

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Purity	≥98%
mp	240-245 °C (dec.) (lit.)
UNSPSC	12352106

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