AccuTaq™ LA DNA Polymerase
SIGMA/D8045 - High fidelity Taq enzyme, with 10X buffer & DMSO
Synonym: High Fidelity DNA polymerase; High fidelity PCR; Long range PCR
MDL Number: MFCD01092095
Product Type: Chemical
Longer products with improved fidelity with AccuTaq LA DNA polymerase Amplification of 2.5, 7, 9, 10, 12, and 15kb fragments of lambda DNA. 2.5 μg of DNA was amplified using 2.5 units of enzyme. The resulting products (6% of total reaction)were electrophoresed on a 0.8% agarose gel. Lanes 3-8 contained standard Taq DNA polymerase and Lanes 11-16 contained AccuTaq LA DNA polymerase. Lanes 1, 9. 1 kb DNA Marker 2, 10. Lambda Hind III DNA Marker 3, 11. 2.5 kb 4, 12. 7 kb
Representative kit contents
| color | colorless |
| concentration | 5 units/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| Long & Accurate PCR | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for PCR |
| technique(s) | PCR: suitable |
| usage | sufficient for 125 reactions |
| sufficient for 1500 reactions | |
| sufficient for 500 reactions |
| Application: | AccuTaq™ LA DNA Polymerase produces long DNA amplicons for: • Mutation analysis • Cloning genes • DNA sequencing • cDNA library generation • Polymerase chain reaction (PCR) • Direct PCR |
| Features and Benefits: | • Increased fidelity, up to 6.5× that of Taq DNA polymerase • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA. |
| General description: | AccuTaq™ long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3’→5’ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end. |
| Legal Information: | AccuTaq is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate. |
| Other Notes: | One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C. |
| Other Notes: | Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance. |
| Packaging: | The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
| Storage Temp. | −20°C |
| UNSPSC | 41106314 |