Deoxynucleotide Mix, 10 mM
SIGMA/D7295 - Molecular Biology Reagent
Synonym: Deoxynucleotide Mix, 10 mM; 10mM dNTP mix; dNTP mix; dNTPs
Product Type: Chemical
Representative kit packaging
| application(s) | agriculture |
| assay | ≥99% |
| biological source | synthetic (organic) |
| color | colorless |
| concentration | 10 mM |
| foreign activity | DNase, RNase, none detected |
| form | liquid |
| Quality Level | 300  |
| shipped in | dry ice |
| solubility | H2O: soluble at 20 °C |
| storage temp. | −20°C |
| Application: | dNTP Mix has been used: • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human, • in reverse transcription of total RNA to cDNA. • as a component of the DNA amplification mixture for polymerase chain reaction (PCR) • routine and long PCR • manual and automated DNA sequencing • cDNA synthesis and labeling reactions |
| Features and Benefits: | • Purity of each dNTP: Minimum 99% • Conveniently formulated; 1 μL is used per 50 μL PCR • Equimolar amounts of each dNTP means less pipetting • Minimize risk of contamination in PCR • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions |
| General description: | Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction. |
| Packaging: | 0.2, 20×0.2, 0.5 mL in poly bottle |
| Packaging: | 25 mL in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |