Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
SIGMA/D6421 - With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: DME/Nutrient Mixture F-12 Ham; DMEM Hams F12; DMEM Hams F12; DME/F-12, 1:1 mixture
MDL Number: MFCD00217417
Product Type: Chemical
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
| components | sodium pyruvate: 0.055 g/L L-glutamine: no phenol red: yes NaHCO3: yes HEPES: 15 mM glucose: 3.15 g/L |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells. |
| Application: | Suitable for growth of wide range of cell types in low serum conditions. Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture. |
| General description: | A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free. |
| Preparation Note: | Supplement with 0.365 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |