Dulbecco’s Modified Eagle’s Medium - low glucose
SIGMA/D5921 - With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym: DME; Dulbecco′s Modified Eagle′s Medium - high glucose; DME; DMEM
MDL Number: MFCD00217342
Product Type: Chemical
| components | phenol red: no sodium pyruvate: no glucose: low HEPES: no L-glutamine: no NaHCO3: yes |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture: • living colon cancer SW620 cells • human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231 • mammal and canine derived adipose-derived mesenchymal stem cells |
| General description: | Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium. |
| Other Notes: | Investigators who work with stem cells or cell clones frequently prefer media without phenol red. |
| Preparation Note: | Supplement with 0.584 g/L |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |