Synonym: 3,3′,4,4′-Biphenyltetramine tetrahydrochloride; 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride; DAB
Empirical Formula (Hill Notation): C12H14N4 · 4HCl
Molecular Weight: 360.11
EC Number: 231-018-9
MDL Number: MFCD00012969
Linear Formula: (NH2)2C6H3C6H3(NH2)2 · 4HCl
Product Type: Chemical
| form |
tablet |
| InChI |
1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H |
| InChI key |
KJDSORYAHBAGPP-UHFFFAOYSA-N |
| Quality Level |
200  |
| SMILES string |
Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2 |
| solubility |
water: 1 tablet/15 mL (may contain small insoluble particles) |
| storage temp. |
−20°C |
| Application: |
3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry. |
| Application: |
Reagent for spectrophotometric determination of selenium. |
| Biochem/physiol Actions: |
3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests. |
| Biochem/physiol Actions: |
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually. |
| Other Notes: |
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Preparation Note: |
Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications. |
| Symbol |
 GHS08 |
| Signal word |
Danger |
| Hazard statements |
H341 - H350 |
| Precautionary statements |
P201 - P308 + P313 |
| Hazard Codes |
T |
| Risk Statements |
45-68 |
| Safety Statements |
53-45 |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Storage Temp. |
−20°C |
| UNSPSC |
12352204 |
