Dulbecco’s Phosphate Buffered Saline
SIGMA/D5652 - Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Synonym: DPBS
MDL Number: MFCD00131855
Product Type: Chemical
Dulbecco′s Phosphate Buffered Saline This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5652-50L
| form | powder |
| pH | 7.2-7.8 |
| Quality Level | 500  |
| solubility | water: 9.6 g/L, clear |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | D-PBS is used to wash cells during preparation and serial transfer. |
| Application: | Dulbecco′s Phosphate Buffered Saline has been used: • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy • to dissolve Angelica gigas powder for microencapsulation • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay • to wash amniotic membrane for cellular analyses |
| Biochem/physiol Actions: | Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range. |
| General description: | Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture. |
| Other Notes: | This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available |
| Preparation Note: | Formulated to contain 9.6 grams of powder per liter of medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |