Dulbecco’s Modified Eagle’s Medium - low glucose
SIGMA/D5546 - With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: DME; Dulbecco′s Modified Eagle′s Medium - high glucose; DME; DMEM
MDL Number: MFCD00217342
Product Type: Chemical
| components | NaHCO3: yes phenol red: yes glucose: low HEPES: no sodium pyruvate: yes L-glutamine: no |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture: • human osteosarcoma cells and HeLa cells • primary human mesenchymal stromal cells (hBMSCs) • human embryonic kidney cell lines |
| General description: | Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. |
| Preparation Note: | Supplement with 0.584 g/L glutamine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |