Deoxyribonuclease I from bovine pancreas
SIGMA/D5025 - Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein
Synonym: DNase I; Deoxyribonucleate 5′-oligonucleotido-hydrolase
CAS Number: 9003-98-9
EC Number: 232-667-0
MDL Number: MFCD00130918
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5025-150KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5025-375KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5025-750KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5025-15KU
| application(s) | diagnostic assay manufacturing diagnostic assay manufacturing |
| biological source | bovine pancreas |
| composition | Protein, ≥80% |
| foreign activity | Chymotrypsin ≤0.5% |
| Protease ≤0.05% | |
| RNase ≤0.02% | |
| form | lyophilized powder |
| mol wt | ~31 kDa |
| purified by | chromatography |
| Quality Level | 300  |
| shipped in | wet ice |
| solubility | 0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless |
| specific activity | ≥2,000 Kunitz units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA purification: suitable |
| type | Type IV |
| Analysis Note: | Protein determined by biuret. |
| Application: | Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I. |
| Application: | DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes. In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids. |
| Application: | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions: | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Other Notes: | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. |
| Packaging: | 15000 units in glass bottle |
| Packaging: | 150000, 375000, 750000 units in poly bottle |
| Physical form: | Lyophilized powder containing calcium chloride |
| Preparation Note: | 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 Kunitz units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |