Dextranase from Penicillium sp.
SIGMA/D4668 - lyophilized powder, 100-250 units/mg protein
Synonym: 1,6-α-D-Glucan 6-glucanohydrolase
CAS Number: 9025-70-1
EC Number: 232-803-9
MDL Number: MFCD00130940
Product Type: Chemical
Dextran is composed of approximately 95% α-D-(1-6) linkages. The remaining α(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000 dextran molecules exhibit the properties of an expandable coil. At MWs below 2,000 dextran is more rod-like.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4668-1KU
| composition | Protein, ~25% Lowry |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 100-250 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure. |
| Biochem/physiol Actions: | The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production. |
| Other Notes: | One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 500, 1000 units in poly bottle |
| Physical form: | Partially purified, lyophilized powder |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 100-250 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |