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Dulbecco’s Modified Eagle’s Medium - low glucose

SIGMA/D2429 - 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

Synonym: DME; Dulbecco′s Modified Eagle′s Medium - high glucose; DME; DMEM

MDL Number: MFCD00217342
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D2429-100ML 100 mL
 $45.50
1/EA		 Add To Favorites
components NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no
concentration 10 ×
form liquid
impurities endotoxin, tested
Quality Level 400 
shipped in ambient
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Dulbecco′s Modified Eagle′s Medium-low glucose has been used:
• during organoid embedding for neutralizing thawed collagen I
• to culture melanoma cancer cell lines
• to store the cancer tissue samples
• as a differentiation culture medium for C2C12 cells

General description: Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.
Preparation Note: Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.
Storage Temp. 2-8°C
UNSPSC 12352207

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