Taq DNA Polymerase from Thermus aquaticus
SIGMA/D1806 - with 10× PCR reaction buffer containing MgCl2
Synonym: MgCl2 taq polymerase; Taq polymerase
CAS Number: 9012-90-2
MDL Number: MFCD00166026
Product Type: Chemical
Representative kit contents
| application(s) | agriculture |
| biological source | enzyme from bacterial (Thermus Aquaticus) |
| color | colorless |
| concentration | 5 units/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| mol wt | 94 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for PCR and automated sequencing reactions |
| technique(s) | PCR: suitable |
| usage | sufficient for 10000 reactions |
| sufficient for 3000 reactions | |
| sufficient for 500 reactions |
| Application: | Taq DNA Polymerase from Thermus aquaticus has been used: • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay • in conventional reverse transcriptase (RT)-PCR • in simple sequence repeats (SSR) genotyping • as a component of PCR mix for amplification of genomic and mitochondrial DNA • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples |
| Biochem/physiol Actions: | Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities. |
| Features and Benefits: | • Low per unit cost of Taq |
| General description: | Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing. |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims. |
| Other Notes: | One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C. |
| Other Notes: | Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity. |
| Packaging: | Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification. |
| Packaging: | Taq DNA Polymerase with 10× reaction buffer containing MgCl2 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |