OptiPrep™ Density Gradient Medium
SIGMA/D1556 - used for cell and subcellular organelle isolation
Synonym: Iodixanol solution
CAS Number: 92339-11-2
MDL Number: MFCD00867965
Product Type: Chemical
| application(s) | hematology histology |
| density | 1.319-1.321 g/mL |
| form | liquid |
| InChI | 1S/C35H44I6N6O15/c1-13(52 |
| InChI key | NBQNWMBBSKPBAY-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | CC(=O)N(CC(O)CN(C(C)=O)c1 |
| sterility | sterile |
| storage temp. | room temp |
| Application: | OptiPrep™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications: • to investigate the efficiency of single-cell encapsulation. • the gradient-based isolation of senescent cancer cells. • isolation of non-enveloped virion isolation from bronchoalveolar lavage. • isolation of lipoproteins and macromolecules • purification of virions • purification of neuron cells |
| Features and Benefits: | OptiPrep™ has numerous biocompatible properties that make it ideal for use. • It is biologically inert and does not interfere with assays for marker enzymes. • It is non-ionic, non-toxic, and has low osmotic pressure. • It forms spontaneous gradients that are stabler and easier to prepare than sucrose. |
| General description: | OptiPrep™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles. |
| Legal Information: | OptiPrep is a trademark of Serumwerk Bernburg AG |
| Other Notes: | 60% (w/v) solution of iodixanol in water (sterile). |
| Packaging: | 250 mL in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.319-1.321 g/mL |
| Storage Temp. | room temp |
| UNSPSC | 12352207 |