DT Diaphorase (NQO1) human
SIGMA/D1315 - lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: DTD; NQO1; Quinone reductase
MDL Number: MFCD00130947
Product Type: Chemical
DT diaphorase (NQO1) is a homodimeric flavoprotein that catalyzes the direct two-electron reduction of quinones such as menadione.
SDS-PAGE DT Diaphorase (NQO1) Human (Cat. No. D1315) was assessed on SDS-PAGE.
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | monomer 31000 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥100 units/mg protein |
| storage temp. | 2-8°C |
| UniProt accession no. | P15559  |
| Application: | Human DT diaphorase has been used in a study to assess the development of novel quinone phosphorodiamidate prodrugs. Human DT diaphorase has also been used to investigate its crystal structure for the development of a model for its interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-din |
| Biochem/physiol Actions: | DT-diaphorase, also referred to as NAD(P)H:(quinone-acceptor |
| Biochem/physiol Actions: | NQO1 is a cytosolic homodimeric FAD-dependent enzyme that catalyses the reduction of a broad range of cytotoxic quinones resulting in protection from cellular oxidative stress. Oxidative stress may also enhance NQO1-mediated protection of p53 and p73 against proteasomal degredation. The highly Inducible expression of NQO1 is controlled by the Nrf2-Keap1/ARE pathway and appears to be affected by changes in susceptibility to oxidative stress. During Oxygen/glucose deprivation, NQO1 appears to be involved in AMPK-induced cancer cell death. NQO1 has been observed to be overexpressed in several types of solid tumors, including breast, pancreas, lung and colon cancer. |
| Biochem/physiol Actions: | Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules. |
| Other Notes: | One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.6.5.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |