1 kb DNA Ladder
SIGMA/D0428 - for DNA electrophoresis
Synonym: 1kb gel ladder; 1kb gel marker; 1kb ladder for gel electrophoresis; DNA gel marker; agarose gel electrophoresis ladder; agarose gel electrophoresis marker
Product Type: Chemical
D0428 on a 0.75% agarose gel
| form | liquid |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| Application: | 1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis. |
| General description: | Sigma’s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520. |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 0.75%. |
| Other Notes: | Sigma’s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |