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Cytochrome c Oxidase Assay Kit

SIGMA/CYTOCOX1 - sufficient for 100 tests, soluble and membrane bound mitochondria

Synonym: Cytochrome c Oxidase Activity Assay Kit

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-CYTOCOX1-1KT 1 kit
 $662.00
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The electron transport protein complex of the inner mitochondrial membrane

 

Quality Level 200 
shipped in dry ice
storage temp. −20°C
usage sufficient for 100 tests
Application: The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Biochem/physiol Actions: Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Features and Benefits: • Simple, optimized protocol - Obtain reproducible results without special training needs
• Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
• Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
• May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
• Use to analyze the intactness of mitochondrial membranes
• Simple colorimetric measurement of solution
Storage Temp. −20°C
UNSPSC 12161503
Components Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mg; n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mg

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