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Glutathione-S-Transferase (GST) Assay Kit

SIGMA/CS0410 - sufficient for 500 multiwell tests

Synonym: GST Enzyme Activity Kit

EC Number: 202-551-4
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-CS0410-1KT 1 kit
 $669.00
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Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
Unit Definition One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

 

Quality Level 200 
shipped in dry ice
storage temp. −20°C
usage sufficient for 500 multiwell tests
Application: The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.
Biochem/physiol Actions: Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.
General description: The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates
Storage Temp. −20°C
UNSPSC 12161503
Components Dulbecco's Phosphate Buffered Saline 100 mL; L-Glutathione Reduced 1 g; GST (Control) .1 mL; Sample Buffer 5 mL; Substrate (CDNB) 1.2 mL

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