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Cell Growth Determination Kit, MTT based

SIGMA/CGD1

Synonym: Mitochondrial activity assay

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-CGD1-1KT 1 kit
 $231.00
1/EA		 Add To Favorites
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Amax 570 nm
application(s) cell analysis
detection
detection method colorimetric
packaging pkg of 1 kit
Quality Level 200 
shipped in dry ice
storage temp. −20°C
technique(s) protein quantification: suitable
  UV/Vis spectroscopy: suitable
Application: Cell Growth Determination Kit, MTT based has been used:
• to measure the cell viability of preosteoblastic cells
• in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
• to determine the cell viability of primary cervical epithelial cells
General description: Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.
Packaging: Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
Storage Temp. −20°C
UNSPSC 12352207

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